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Indeed, the clinical literature exhibits heterogeneity in design, dosing, duration, and outcome measures, frequent reliance on parent reported scales without uniform blinding, and underpowered samples that hinder strong inference and guideline development (Doherty et al., 2022

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Following this, cell media containing 143B cells at a density of 3 × 10 5 were added and incubated overnight in a cell culture incubator