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To further explore the regulatory function of glutamic acid and aspartic acid in vasodilation, we treated human dermal microvascular endothelial cells (HDMECs) with glutamic acid, aspartic acid, and leucine and found that glutamic acid and aspartic acid increased the phosphorylation of eNOS in a dose-dependent manner (Figure 4D), but leucine did not (Supplemental Figure 3A)