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In Arabidopsis , PRR-Associated Kinase BIK1 directly phosphorylates NADPH oxidase RBOHD and causes the PAMP-induced ROS burst and antibacterial immunity (Kadota et al., 2014)

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Hydrophobic residues (such as Leu and Ala) cover the surface of the active cavity, which reduces the hydrophobic complementarity between the enzyme and the substrate, and further inhibits the catalytic efficiency (Hu et al., 2022b)