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31 P NMR sample preparation and data acquisition For KRAS-Q61R·GppNHp preparation, the KRAS-Q61R after anion-exchange column as described above was diluted in reaction buffer (5 mM HEPES, pH 7.5, 150 mM NaCl, 5 mM EDTA, 1 mM DTT) to a final concentration of 315 µM KRAS-Q61R, 21.3 mg GppNHp (final concentration 7.78 mM) was added