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Size:10mg/10mg/50mg (one vial)

Cells were seeded on low-attachment culture vessels (Corning) and cultured in mTeSR1 medium supplemented with 40 μg ml −1 Matrigel, 1 ng ml −1 BMP4 (Invitrogen) and 10 μM Rho kinase inhibitor (ROCK) (R&D) under hypoxic environment with 5% O 2