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The procedure itself is quick, typically taking about 30 minutes

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Samples were separated using a Kinetex ® 2.6 μm XB-C18 100 Å, 100 × 2.1 mm LC Column (Phenomenex) with a constant flow rate of 250 μl/min following a flow gradient starting at 70% Mobile Phase B (ACN + 0.5% Acetic acid, AA) then going up to 99% for 5 min before re-equilibration at 70% for 5 min