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[DOI] [PMC free article] [PubMed] [Google Scholar] 10.Almeida S., Ozkan S., Gonçalves D., Paulo I., Queirós C.S.G.P., Ferreira O., Bordado J., Galhano dos Santos R

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After a defined incubation period, the CUPRAC reagent Cu(Nc) 2 2+ is added to stop enzymatic reactions, allowing unreacted substrates to reduce the Cu(II)-neocuproine complex to the intensely colored Cu(I)-neocuproine complex, which exhibits maximum absorbance at 450 nm