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Immunofluorescence staining Fibroblasts were fixed, permeabilised, blocked, and incubated overnight at 4 °C with primary antibodies targeting p53-pS15 and FOXO4 shown in Table 2, and developed using Alexa Fluor 488-conjugated donkey anti-mouse immunoglobulin G (1:100

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Most users notice side effects within 24 to 48 hours after their weekly dose, but the acute peak, that first wave of queasiness, typically arrives within 1 to 3 hours post-injection